Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy

Cell-based immunotherapy using natural killer (NK) cells, cytokine-induced killer (CIK) cells and dendritic cells (DCs) is emerging as a potential novel approach in the auxiliary treatment of a tumor. However, non-standard operation procedure, small-scale cell number, or human error may limit the cl...

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Main Authors: Yiming Meng (Author), Jing Sun (Author), Tingting Hu (Author), Yushu Ma (Author), Tiaozhao Du (Author), Cuicui Kong (Author), Guirong Zhang (Author), Tao Yu (Author), Haozhe Piao (Author)
Format: Book
Published: Taylor & Francis Group, 2018-10-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Yiming Meng  |e author 
700 1 0 |a Jing Sun  |e author 
700 1 0 |a Tingting Hu  |e author 
700 1 0 |a Yushu Ma  |e author 
700 1 0 |a Tiaozhao Du  |e author 
700 1 0 |a Cuicui Kong  |e author 
700 1 0 |a Guirong Zhang  |e author 
700 1 0 |a Tao Yu  |e author 
700 1 0 |a Haozhe Piao  |e author 
245 0 0 |a Rapid expansion in the WAVE bioreactor of clinical scale cells for tumor immunotherapy 
260 |b Taylor & Francis Group,   |c 2018-10-01T00:00:00Z. 
500 |a 2164-5515 
500 |a 2164-554X 
500 |a 10.1080/21645515.2018.1480241 
520 |a Cell-based immunotherapy using natural killer (NK) cells, cytokine-induced killer (CIK) cells and dendritic cells (DCs) is emerging as a potential novel approach in the auxiliary treatment of a tumor. However, non-standard operation procedure, small-scale cell number, or human error may limit the clinical development of cell-based immunotherapy. To simplify clinical scale NK cells, CIK cells and DCs expansions, we investigated the use of the WAVE bioreactor, a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes. We developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant number of cells for our adoptive cell transfer clinical protocols. The high proliferative rate, surface phenotypes, and cytotoxicity of these immune cells, as well as the safety of cultivation were analyzed to illuminate the effect of WAVE bioreactor. The results demonstrated that the benefit of utilizing modern WAVE bioreactors in cancer immunotherapy was simple, safe, and flexible production. 
546 |a EN 
690 |a tumor immunotherapy 
690 |a nk cells 
690 |a cik cells 
690 |a dcs 
690 |a wave bioreactor 
690 |a Immunologic diseases. Allergy 
690 |a RC581-607 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Human Vaccines & Immunotherapeutics, Vol 14, Iss 10, Pp 2516-2526 (2018) 
787 0 |n http://dx.doi.org/10.1080/21645515.2018.1480241 
787 0 |n https://doaj.org/toc/2164-5515 
787 0 |n https://doaj.org/toc/2164-554X 
856 4 1 |u https://doaj.org/article/170b9c3f4e3d4e3e8bdd612eeed6119c  |z Connect to this object online.