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A Trypsin Inhibitor from <i>Moringa oleifera</i> Flowers Modulates the Immune Response In Vitro of <i>Trypanosoma cruzi</i>-Infected Human Cells

<i>Trypanosoma cruzi</i> causes the lethal Chagas disease, which is endemic in Latin America. Flowers of <i>Moringa oleifera</i> (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to <i>T. cruzi</i> trypomastigotes was previously reported. Here, we s...

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Main Authors: Isabella Coimbra Vila Nova (Author), Leyllane Rafael Moreira (Author), Diego José Lira Torres (Author), Kamila Kássia dos Santos Oliveira (Author), Leydianne Leite de Siqueira Patriota (Author), Luana Cassandra Breitenbach Barroso Coelho (Author), Patrícia Maria Guedes Paiva (Author), Thiago Henrique Napoleão (Author), Virgínia Maria Barros de Lorena (Author), Emmanuel Viana Pontual (Author)
Format: Book
Published: MDPI AG, 2020-08-01T00:00:00Z.
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Summary:<i>Trypanosoma cruzi</i> causes the lethal Chagas disease, which is endemic in Latin America. Flowers of <i>Moringa oleifera</i> (Moringaceae) express a trypsin inhibitor (MoFTI) whose toxicity to <i>T. cruzi</i> trypomastigotes was previously reported. Here, we studied the effects of MoFTI on the viability of human peripheral blood mononuclear cells (PBMCs) as well as on the production of cytokines and nitric oxide (NO) by <i>T. cruzi</i>-infected PBMCs. Incubation with MoFTI (trypsin inhibitory activity: 62 U/mg) led to lysis of trypomastigotes (LC<sub>50</sub> of 43.5 µg/mL) but did not affect the viability of PBMCs when tested at concentrations up to 500 µg/mL. A selectivity index > 11.48 was determined. When <i>T. cruzi</i>-infected PBMCs were treated with MoFTI (43.5 or 87.0 µg/mL), the release of the pro-inflammatory cytokine TNF-α and INF-γ, as well as of NO, was stimulated. The release of the anti-inflammatory cytokine IL-10 also increased. In conclusion, the toxicity to <i>T. cruzi</i> and the production of IL-10 by infected PBMCs treated with MoFTI suggest that this molecule may be able to control parasitemia while regulating the inflammation, preventing the progress of Chagas disease. The data reported here stimulate future investigations concerning the in vivo effects of MoFTI on immune response in Chagas disease.
Item Description:10.3390/antibiotics9080515
2079-6382

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