<i>Andrographis paniculata</i> and Its Bioactive Diterpenoids Against Inflammation and Oxidative Stress in Keratinocytes

• Автори: Eugenie Mussard (Автор), Sundy Jousselin (Автор), Annabelle Cesaro (Автор), Brigitte Legrain (Автор), Eric Lespessailles (Автор), Eric Esteve (Автор), Sabine Berteina-Raboin (Автор), Hechmi Toumi (Автор)
• Формат: Книга
• Опубліковано: MDPI AG, 2020-06-01T00:00:00Z.
• Предмети: article
• Онлайн доступ: Connect to this object online.

Резюме

<i>Andrographis paniculata</i> was widely used in traditional herbal medicine to treat various diseases. This study explored the potential anti-aging activity of <i>Andrographis paniculata</i> in cutaneous cells. Human, adult, low calcium, high temperature (HaCaT) cells were treated with methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12). Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by fluorescence using a 2',7'-dichlorofluorescein diacetate (DCFH-DA) probe and cytokines were quantified by ELISA for interleukin-8 (IL-8) or reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for tumor necrosis factor-α (TNF-α). Hyaluronic acid (HA) secretion was determined by an ELISA. Our results show a decrease in ROS production and TNF-α expression by ME (5 µg/mL) in HaCaT under pro-oxidant and pro-inflammatory conditions, respectively. ME protected HaCaT against oxidative stress and inflammation. Our findings confirm that ME can be used for the development of bioactive compounds against epidermal damage.

Опис примірника

10.3390/antiox9060530
2076-3921