Chemotactic Activity on Human Neutrophils to Streptococcus mutans
<p><strong>Objective: </strong>The aim of this study was to evaluate chemotactic activity o neutrophil to S. mutans. Chemotaxis assay was performed in blind well chambers.<strong> Materials and Methods:</strong> Hanks balanced salt solution (HBSS) containing 10<sup&g...
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Faculty of Dentistry, Universitas Indonesia,
2013-07-01T00:00:00Z.
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| Summary: | <p><strong>Objective: </strong>The aim of this study was to evaluate chemotactic activity o neutrophil to S. mutans. Chemotaxis assay was performed in blind well chambers.<strong> Materials and Methods:</strong> Hanks balanced salt solution (HBSS) containing 10<sup>6 </sup>S. mutans, 10<sup>8 </sup>S. mutans, 10<sup>-8 </sup>M fMLP, or HBSS alone were placed in the lower wells of the chamber and covered with polycorbonate membrane filter. Neutrophils suspension (2x10<sup>5 </sup>cells) was then placed in the upper compartment. After incubation for 60 mins at 37ºC in a humidified atmosphere with 5% CO<sub>2</sub>, the filters were removed and stained with Giemsa. <strong>Result: </strong>ANOVA revealed statistically significant differences among groups (p<0.05), indicating that S. mutans induced neutrophils chemotaxis. The number of neutrophils migration in response to 10<sup>8 </sup>S. mutans and 10<sup>6 </sup>S. mutans were signifiantly greater compared to fMLP (p<0.05). <strong>Conclusion:</strong> S. mutans may activate human neutrophils, resulting in the chemotaxis of the neutrophils.</p><p>DOI: 10.14693/jdi.v16i2.99</p> |
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| Item Description: | 1693-9697 2355-4800 10.14693/jdi.v16i2.99 |