Epidemiology and molecular identification of mixed yeast isolates in Malaysia- A way forward
Background and Purpose: Invasive candidiasis is one of the most common systemicmycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeastculture data.Materials and Methods: Yeast isolates were collected in Sultan Abdul Halim Hospital,North Malaysia, from October 2020 to Octob...
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| Main Authors: | , , , , , |
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| Format: | Book |
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Mazandaran University of Medical Sciences,
2022-09-01T00:00:00Z.
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| Summary: | Background and Purpose: Invasive candidiasis is one of the most common systemicmycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeastculture data.Materials and Methods: Yeast isolates were collected in Sultan Abdul Halim Hospital,North Malaysia, from October 2020 to October 2021. Chromogenic Candida differentialagar media and PCR-RFLP were used to identify yeast species.Results: A total of 206 yeast isolates were collected from different body sites of patients.The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of Candida species.Conclusion: Malaysia lacks accurate epidemiological data on mixed yeast infections.We identified all samples to the species level, including mixed yeast cultures, using theMspI enzyme and PCR-RFLP. |
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| Item Description: | 2423-3439 2423-3420 10.18502/cmm.8.3.11209 |