Ether Derivatives of Naringenin and Their Oximes as Factors Modulating Bacterial Adhesion

• Main Authors: Anna Duda-Madej (Author), Joanna Kozłowska (Author), Dagmara Baczyńska (Author), Paweł Krzyżek (Author)
• Format: Book
• Published: MDPI AG, 2023-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antibiotics12061076; 2079-6382
• Keywords: adhesion; biofilm; Bioflux; microfluidic conditions; naringenin derivatives; intestinal microflora; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 12, Iss 6, p 1076 (2023)

Because of the close connection between adhesion and many vital cellular functions, the search for new compounds modulating the adhesion of bacteria belonging to the intestinal microbiota is a great challenge and a clinical need. Based on our previous studies, we discovered that <i>O</i>-lkyl naringenin derivatives and their oximes exhibit antimicrobial activity against antibiotic-resistant pathogens. The current study was aimed at determining the modulatory effect of these compounds on the adhesion of selected representatives of the intestinal microbiota: <i>Escherichia coli</i>, a commensal representative of the intestinal microbiota, and <i>Enterococcus faecalis</i>, a bacterium that naturally colonizes the intestines but has disease-promoting potential. To better reflect the variety of real-life scenarios, we performed these studies using two different intestinal cell lines: the physiologically functioning ("healthy") 3T3-L1 cell line and the disease-mimicking, cancerous HT-29 line. The study was performed in vitro under static and microfluidic conditions generated by the Bioflux system. We detected the modulatory effect of the tested <i>O</i>-alkyl naringenin derivatives on bacterial adhesion, which was dependent on the cell line studied and was more significant for <i>E. coli</i> than for <i>E. faecalis</i>. In addition, it was noticed that this activity was affected by the concentration of the tested compound and its structure (length of the carbon chain). In summary, <i>O</i>-alkyl naringenin derivatives and their oximes possess a promising modulatory effect on the adhesion of selected representatives of the intestinal microbiota.