Comprehensive engineering of a therapeutic neutralizing antibody targeting SARS-CoV-2 spike protein to neutralize escape variants

• Main Authors: Taichi Kuramochi (Author), Siok Wan Gan (Author), Adrian W.S. Ho (Author), Bei Wang (Author), Nagisa Kageji (Author), Takeru Nambu (Author), Sayaka Iida (Author), Momoko Okuda-Miura (Author), Wei Shan Chia (Author), Chiew Ying Yeo (Author), Dan Chen (Author), Wen-Hsin Lee (Author), Eve Zi Xian Ngoh (Author), Siti Nazihah Mohd Salleh (Author), Cheng-I Wang (Author), Tomoyuki Igawa (Author), Hideaki Shimada (Author)
• Format: Book
• Published: Taylor & Francis Group, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The emergence of escape variants of SARS-CoV-2 carrying mutations in the spike protein poses a challenge for therapeutic antibodies. Here, we show that through the comprehensive engineering of the variable region of the neutralizing monoclonal antibody 5A6, the engineered antibody, 5A6CCS1, is able...