ESTIMATION OF METHIONINE AND LYSINE IN PROTEIN BY THIN-LAYER CHROMATOGRAPHY
A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyrid...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Published: |
Tehran University of Medical Sciences,
1983-12-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. Procedures in all the three stages were in the same dimension. Results of casein analysis by this method showed ± 15% fluctuations when compared with standard amino acid analyzer. |
|---|---|
| Item Description: | 2251-6085 2251-6093 |