The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons

• Autores principales: He-Bin Tang (Autor), Yu-Sang Li (Autor), Yoshihiro Nakata (Autor)
• Formato: Libro
• Publicado: Elsevier, 2007-01-01T00:00:00Z.
• Materias: article
• Acceso en línea: Connect to this object online.
• Notes: 1347-8613; 10.1254/jphs.FP0071359
• Keywords: Therapeutics. Pharmacology; RM1-950
• Source: Journal of Pharmacological Sciences, Vol 105, Iss 3, Pp 264-271 (2007)

Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium-sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells. Keywords:: capsaicin, dorsal root ganglion (DRG) neuron, non-neuronal cell, substance P content, substance P release