<it>Piper betle</it> shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase

<p>Abstract</p> <p>Background</p> <p>Breast cancer is the most common form of cancer and the focus on finding chemotherapeutic agents have recently shifted to natural products. <it>Piper betle</it> is a medicinal plant with various biological activities. How...

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Main Authors: Abrahim Noor (Author), Kanthimathi M S (Author), Abdul-Aziz Azlina (Author)
Format: Book
Published: BMC, 2012-11-01T00:00:00Z.
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001 doaj_1b09f893f21a435a93faa55a9ef7d530
042 |a dc 
100 1 0 |a Abrahim Noor  |e author 
700 1 0 |a Kanthimathi M S  |e author 
700 1 0 |a Abdul-Aziz Azlina  |e author 
245 0 0 |a <it>Piper betle</it> shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase 
260 |b BMC,   |c 2012-11-01T00:00:00Z. 
500 |a 10.1186/1472-6882-12-220 
500 |a 1472-6882 
520 |a <p>Abstract</p> <p>Background</p> <p>Breast cancer is the most common form of cancer and the focus on finding chemotherapeutic agents have recently shifted to natural products. <it>Piper betle</it> is a medicinal plant with various biological activities. However, not much data is available on the anti-cancer effects of <it>P. betle</it> on breast cancer. Due to the current interest in the potential effects of antioxidants from natural products in breast cancer treatment, we investigated the antioxidant activities of the leaves of <it>P. betle</it> and its inhibitory effect on the proliferation of the breast cancer cell line, MCF-7.</p> <p>Methods</p> <p>The leaves of <it>P. betle</it> were extracted with solvents of varying polarities (water, methanol, ethyl acetate and hexane) and their phenolic and flavonoid content were determined using colorimetric assays. Phenolic composition was characterized using HPLC. Antioxidant activities were measured using FRAP, DPPH, superoxide anion, nitric oxide and hyroxyl radical scavenging assays. Biological activities of the extracts were analysed using MTT assay and antioxidant enzyme (catalase, superoxide dismutase, glutathione peroxidase) assays in MCF-7 cells.</p> <p>Results</p> <p>Overall, the ethyl acetate extract showed the highest ferric reducing activity and radical scavenging activities against DPPH, superoxide anion and nitric oxide radicals. This extract also contained the highest phenolic content implying the potential contribution of phenolics towards the antioxidant activities. HPLC analyses revealed the presence of catechin, morin and quercetin in the leaves. The ethyl acetate extract also showed the highest inhibitory effect against the proliferation of MCF-7 cells (IC<sub>50</sub>=65 μg/ml). Treatment of MCF-7 cells with the plant extract increased activities of catalase and superoxide dismutase.</p> <p>Conclusions</p> <p>Ethyl acetate is the optimal solvent for the extraction of compounds with antioxidant and anti-proliferative activities. The increased activities of catalase and superoxide dismutase in the treated cells could alter the antioxidant defense system, potentially contributing towards the anti-proliferative effect. There is great potential for the ethyl acetate extract of <it>P. betle</it> leaf as a source of natural antioxidants and to be developed as therapeutics in cancer treatment.</p> 
546 |a EN 
690 |a <it>Piper betle</it> 
690 |a Antioxidant 
690 |a Phenolic 
690 |a MCF-7 
690 |a Cytotoxicity 
690 |a Catalase 
690 |a Superoxide dismutase 
690 |a HPLC 
690 |a Other systems of medicine 
690 |a RZ201-999 
655 7 |a article  |2 local 
786 0 |n BMC Complementary and Alternative Medicine, Vol 12, Iss 1, p 220 (2012) 
787 0 |n http://www.biomedcentral.com/1472-6882/12/220 
787 0 |n https://doaj.org/toc/1472-6882 
856 4 1 |u https://doaj.org/article/1b09f893f21a435a93faa55a9ef7d530  |z Connect to this object online.