Reactive oxygen species (ROS) generation by lymphocytes in rats treated with a common food additive E407a
This research deals with the evaluation of reactive oxygen species (ROS) generation in lymphocytes of rats orally exposed to a thickener and emulsifier E407a (semi-refined carrageenan). There is some evidence that E407a may be toxic and can induce intestinal inflammation. ROS generation in lymphocyt...
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National Scientific Medical Center,
2020-03-01T00:00:00Z.
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| Summary: | This research deals with the evaluation of reactive oxygen species (ROS) generation in lymphocytes of rats orally exposed to a thickener and emulsifier E407a (semi-refined carrageenan). There is some evidence that E407a may be toxic and can induce intestinal inflammation. ROS generation in lymphocytes was assessed by flow cytometry using a dye 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) in 8 rats treated with 140 mg of semi-refined carrageenan per kg of weight daily during two weeks and 8 control animals. A higher number of ROS<sup>high</sup> viable lymphocytes were found in the group of animals exposed to E407a compared with controls. In addition, mean fluorescence intensity (MFI) in ROS<sup>high</sup> lymphocytes was elevated, which is indicative of the intensification of ROS production by lymphocytes in response to E407a oral consumption. |
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| Item Description: | 1812-2892 2313-1519 10.23950/1812-2892-JCMK-00744 |