The silence of MUC2 mRNA induced by promoter hypermethylation associated with HBV in Hepatocellular Carcinoma

• Main Authors: Ling Yang (Author), Zhu Jing (Author), Gao Lu (Author), Liu Yongping (Author), Zhu Changtai (Author), Li Rong (Author), Wei Lixin (Author), Zhang Changsong (Author)
• Format: Book
• Published: BMC, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>To evaluate the promoter methylation status of MUC2 gene and mRNA expression in patients with hepatocellular carcinoma.</p> <p>Methods</p> <p>We analyzed MUC2 methylation by MSP, and MUC2 mRNA by real-time PCR in 74 HCC.</p> <p>Results</p> <p>MUC2 mRNA were lower in HCC tissues (Mean _-ΔCt = −4.70) than that in Non-HCC tissues (Mean _-ΔCt = −2.98). Expression of MUC2 was elevated in only 23 (31.08%) of the 74 HCC patients. MUC2 promoter was hypermethylated in 62.2% (46/74) of HCCs, and in only 18.9% (14/74) of non-tumor samples. MUC2 mRNA were lower in HCC patients with hypermethylation (Mean _-ΔΔCt = −2.25) than those with demethylation (Mean _-ΔΔCt = −0.22), and there is a decreased tendency for MUC2 mRNA in HCC patients with promoter hypermethylation (<it>p</it> = 0.011). There was a significantly correlation found between MUC2 mRNA and HBV and AFP in HCC. The loss of MUC2 mRNA and hypermethylation could be poor prognostic factors. After treated by 5-Aza-CdR and TSA, we found that MUC2 mRNA induced significantly in 7721, Huh7 and HepG2 cells.</p> <p>Conclusion</p> <p>The results suggested that MUC2 mRNA silenced by promoter hypermethylation is associated with high levels HBV in HCC.</p>

Item Description

10.1186/1471-2350-14-14
1471-2350