Andrographolide Inhibits Static Mechanical Pressure-Induced Intervertebral Disc Degeneration via the MAPK/Nrf2/HO-1 Pathway

• Main Authors: Zhang C (Author), Lu Z (Author), Lyu C (Author), Zhang S (Author), Wang D (Author)
• Format: Book
• Published: Dove Medical Press, 2023-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Cunxin Zhang,1,2 Ziang Lu,3 Chaoliang Lyu,2 Shanshan Zhang,4 Dechun Wang1 1Department of Spine Surgery, Qingdao Municipal Hospital, Shandong University, Qingdao, 266061, People's Republic of China; 2Department of Spine Surgery, Jining No.1 People's Hospital, Jining, 272011, People's Republic of China; 3Jining Medical University, Jining, 272067, People's Republic of China; 4Department of Neurology, Jining No.1 People's Hospital, Jining, 272011, People's Republic of ChinaCorrespondence: Dechun Wang, Department of Spine surgery, Qingdao Municipal Hospital, Shandong University, 5# Donghai Road, Shinan District, Qingdao, 266061, People's Republic of China, Tel +86+18661809296, Fax [phone], Email [e-mail]: To explore the molecular mechanism by which andrographolide (ADR) inhibits static mechanical pressure-induced apoptosis in nucleus pulposus cells (NPCs) and to assess the role of ADR in inhibiting IDD.Methods: Hematoxylin-eosin (HE), toluidine blue, and immunofluorescence staining were used to identify NPCs. An NPC apoptosis model was constructed using a homemade cell pressurization device. The proliferation activity, reactive oxygen species (ROS) content, and apoptosis rate were detected using kits. The expression of related proteins was detected using Western blot. A rat tailbone IDD model was constructed using a homemade tailbone stress device. HE staining and safranine O-fast green FCF cartilage staining were used to observe the degeneration degree of the intervertebral disk.Results: ADR inhibits static mechanical pressure-induced apoptosis and ROS accumulation in NPCs and improves cell viability. ADR can promote the expression of Heme oxygenase-1 (HO-1), p-Nrf2, p-p38, p-Erk1/2, p-JNK, and other proteins, and its effects can be blocked by inhibitors of the above proteins.Conclusion: ADR can inhibit IDD by activating the MAPK/Nrf2/HO-1 signaling pathway and suppressing static mechanical pressure-induced ROS accumulation in the NPCs.Keywords: andrographolide, intervertebral disc degeneration, nucleus pulposus cells, apoptosis, reactive oxygen species, HO-1

Item Description

1177-8881