Aktivitas antibakteri flavonoid propolis Trigona sp terhadap bakteri Streptococcus mutans (in vitro) (In vitro antibacterial activity of flavonoids Trigona sp propolis against Streptococcus mutans)
<span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A number of investigations have shown a positive correlation between the number of Streptococcus mutans (S. mutans) in dental </em><span style="...
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Universitas Airlangga,
2005-09-01T00:00:00Z.
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| Summary: | <span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A number of investigations have shown a positive correlation between the number of Streptococcus mutans (S. mutans) in dental </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>plaque and the prevalence of dental caries. Consequently, this microorganism has been the prime target for the prevention of dental </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>caries. Propolis being a substance made by the honeybee, is a potent antibacterial agent. The main chemical class present in </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>propolis is flavonoids. Flavonoids are well-known plant compounds that have antibacterial property. Because S. mutans is accepted </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>to be one of the microorganisms responsible for dental caries and flavonoids in propolis are antibacterial, the purpose of this study </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>was to evaluate in vitro the antibacterial activity of flavonoids Trigona sp propolis against S. mutans as a first step in its possible </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>use as an alternative anticaries agent. Extract flavonoids was purified from ethanol extract of propolis which was obtained from </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>Bulukumba Regency South Sulawesi using thin layer chromatography. The purification of flavonoids was carried-out by UVradiation at </em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">λ <span style="font-family: TribuneItalic; font-size: 7pt; color: #000000; font-style: normal; font-variant: normal;"><em>max </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>254 nm and </em><span style="font-family: SymbolProportionalBT-Regular; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">λ<span style="font-family: TribuneItalic; font-size: 7pt; color: #000000; font-style: normal; font-variant: normal;"><em>max </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>366 nm and treatment with ammonia. Extract flavonoids was diluted in aquadest to 0.05%; </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>0.075%; 0.1%; 0.25%; 0.5%; 0.75% concentrations. Aquadest and 10% Povidone iodine were also used as control solution. S </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>mutans were grown in medium glucose nutrient agar and incubated with flavonoids for 24 and 48 hours, at 37° C. Antibacterial </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>activity was reflected by the diameter of the inhibition zones around the stainless steel cylinder. The data were analyzed by using </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>ANOVA followed by LSD test with significance level of 5%. The results of this study showed that after being incubated for 24 and 48 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>hours, all flavonoid concentrations significantly (p < 0.05) inhibited the growth of S mutans. 0.1% flavonoid was the most effective </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>concentration to inhibit the growth of S mutans after 24 hours of incubation and 0.5% flavonoid after 48 hours of incubator.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> |
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| Item Description: | 1978-3728 2442-9740 10.20473/j.djmkg.v38.i3.p135-141 |