Evaluation of the Diuretic Activity of Aqueous and 80% Methanol Extracts of Croton macrostachyus(Euphorbiaceae) Leaves in Saline-Loaded Rats

• Autori principali: Tufer S (Autore), Engidawork E (Autore), Ayele AG (Autore), Bashea C (Autore)
• Natura: Libro
• Pubblicazione: Dove Medical Press, 2021-03-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.
• Notes: 1179-1454
• Keywords: croton macrostachuys del; diuretic activity; electrolyte; leaf; rats; Therapeutics. Pharmacology; RM1-950
• Source: Journal of Experimental Pharmacology, Vol Volume 13, Pp 213-221 (2021)

Sara Tufer,1 Ephrem Engidawork,1 Akeberegn Gorems Ayele,1 Chala Bashea2 1Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaCorrespondence: Akeberegn Gorems AyeleDepartment of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Zambia Street, PO Box 9086, Addis Ababa, EthiopiaTel [phone]Email [e-mail]: Croton macrostachyus (Euphorbiaceae) extract is a folk medicine traditionally used for treating a number of disorders, including edematous conditions. The present study aimed to evaluate the diuretic effects of aqueous and 80% methanol leaf extracts of Croton macrostachyus in saline-loaded rats.Methods: Rats of either sex were randomly assigned into eight groups of eight animals per group. The animals were treated with vehicle (distilled water), standard (furosemide 10 mg/kg), and three doses (100, 200, and 400 mg/kg) of aqueous and 80% methanol leaf extracts after loading of normal saline (15 mL/kg). Then, urine volume, electrolyte concentration, and pH were measured as parameters of evaluation. Concentrations of urinary Na+ K+, and Cl– were determined and Na+:K+ and Cl−:Na+ + K+ ratios calculated to reveal possible mechanisms.Results: The aqueous extract at 200 mg/kg had produced significant diuresis by hour 3, while the same dose of 80% methanol extract had produced substantial diuresis by the end of hour 4. Both extracts at 400 mg/kg produced significant diuresis from hour 2 to hour 5. In terms of effect on electrolysis, 400 mg/kg aqueous extract produced significant natriuresis, and a kaliuresis effect was observed for both extracts at higher doses and 200 mg/kg aqueous extract.Conclusion: The findings collectively indicated that both aqueous and 80% methanol extract showed significant diuretic activity, thereby justifying the plant’s traditional use as a diuretic agent.Keywords: Croton macrostachyus, diuretic activity, electrolyte, leaf, rats