Perbandingan sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas (Citotoxicity comparation of three types of algyrogel on fibroblast cells)

• Main Authors: Stefany Elan Saktiyawardani (Author), Hardono Jaya Lauson (Author), Anugerah Pekerti Astamurtiningrum (Author), Mahadna Aulia Rahmah (Author), Pramana Pananja Putra (Author), Juni Handajani (Author)
• Format: Book
• Published: Universitas Airlangga, 2014-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The prevalence of osteoporosis and bone defects in the world and Indonesia increase each year. Algyrogel is a </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>alginate-based hydrogel material recently very popular used as a bone substitute through injectable bone substitute (IBs) method. One </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of advantages algyrogel is biocompatibility to the body tissues. some natural-based polymer (alginate, chitosan, dan hyaluronat) have </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>different biocompatibility. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The study was aimed to determine the cytotoxicity of three types algyrogel against fibroblast cells. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This study used three types algyrogel, there were algyrogel containing alginate (Alg), a mixture of alginate with chitosan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(Alg/Ch), and a mixture of alginate with hyaluronic acid (Alg/Ha). Each type algyrogel was exposed in the Vero cell line of fibroblast </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>with volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; and 20 μL respectively. The cytotoxicity performed by MTT </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>method to determine the percentage of cell death. The optical density was measured by an ELIsA plate reader then data was analyzed </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>using ANOVA and probit. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The percentage of cell death in all groups of algyrogel have below 30%. LC50 value at Alg was </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>651.017 mg/mL (21.56%), Alg/Ch was 280.478 mg/mL (20.91%), and Alg/Ha was 1054.094 mg/ mL (17.2%). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Alg/Ha has </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the lowest cytotoxicity on fibroblast cells and may have potential as an osteoconductor-synthetic bone product.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Prevalensi osteoporosis dan cacat tulang di Indonesia maupun dunia semakin meningkat tiap tahunnya. Algyrogel </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sebagai bahan hidrogel yang berbasis alginat akhir-akhir ini sangat popular digunakan sebagai bahan pengganti tulang melalui metode </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Injectable Bone substitute. Kelebihan algyrogel antara lain biokompatibel terhadap jaringan tubuh. Beberapa hidrogel berbasis polimer </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>alami (alginat, chitosan, dan hyaluronat) memiliki perbedaan biokompatibilitas. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>studi ini bertujuan meneliti sitotoksisitas </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>tiga jenis algyrogel terhadap sel fibroblas. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini menggunakan 3 jenis algyrogel yaitu algyrogel dengan kandungan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>alginat (Alg), campuran alginat dengan chitosan (Alg/Ch), dan campuran alginat dengan asam hyaluronat (Alg/Ha).
setiap jenis </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>algyrogel dipaparkan pada sel fibroblas jenis Vero cell line dengan volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dan 20 μL. Pengujian sitotoksisitas dilakukan dengan metode MTT untuk mengetahui persentase kematian sel. Penghitungan optical </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>density menggunakan ELIsA plate reader lalu data diuji Anova dan probit. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Persentase kematian sel pada semua jenis algyrogel </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>memiliki angka dibawah 30%. Nilai LC</em><span style="font-family: Tribune-Italic; font-size: 7pt; color: #231f20; font-style: normal; font-variant: normal;"><em>50 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>pada Alg sebesar 651,017 μg/μL (21,56%), Alg/Ch sebesar 280,478 μg/μL (20.91%), dan</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> "> <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Alg/Ha sebesar 1054,094 μg/μL (17,2%). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Alg/Ha memiliki sitotoksisitas paling rendah pada sel fibroblas sehingga memiliki </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>potensi sebagai produk osteokonduktor sintesis tulang.</em></span></span></span></span><br /></em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v47.i3.p130-134