Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
ABSTRACT The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are s...
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| Main Authors: | , , , |
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| Format: | Book |
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Universidade de São Paulo,
2018-07-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_1c57bb3fd3fc40f5a95eafd7f3bda77c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Carla Lilian de Agostini Utescher |e author |
| 700 | 1 | 0 | |a Klariane Laís Buosi |e author |
| 700 | 1 | 0 | |a Viviane Fungaro Botosso |e author |
| 700 | 1 | 0 | |a Wagner Quintilio |e author |
| 245 | 0 | 0 | |a Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera |
| 260 | |b Universidade de São Paulo, |c 2018-07-01T00:00:00Z. | ||
| 500 | |a 2175-9790 | ||
| 500 | |a 10.1590/s2175-97902018000217530 | ||
| 520 | |a ABSTRACT The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation. | ||
| 546 | |a EN | ||
| 690 | |a Monocytes Activation Test | ||
| 690 | |a Pyrogen | ||
| 690 | |a Hyperimmune serum | ||
| 690 | |a Pyrodetect kit | ||
| 690 | |a Cryoblood. | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Brazilian Journal of Pharmaceutical Sciences, Vol 54, Iss 2 (2018) | |
| 787 | 0 | |n http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200605&lng=en&tlng=en | |
| 787 | 0 | |n https://doaj.org/toc/2175-9790 | |
| 856 | 4 | 1 | |u https://doaj.org/article/1c57bb3fd3fc40f5a95eafd7f3bda77c |z Connect to this object online. |