Re-Use of Caco-2 Monolayers in Permeability Assays-Validation Regarding Cell Monolayer Integrity

• Main Authors: Cristiana L. Pires (Author), Catarina Praça (Author), Patrícia A. T. Martins (Author), Ana L. M. Batista de Carvalho (Author), Lino Ferreira (Author), Maria Paula M. Marques (Author), Maria João Moreno (Author)
• Format: Book
• Published: MDPI AG, 2021-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Caco-2 monolayers are a common in vitro model used to evaluate human intestinal absorption. The reference protocol requires 21 days post-seeding to establish a stable and confluent cell monolayer, which is used in a single permeability assay during the period of monolayer stability (up to day 30). I...