Antioxidant Activity and Acteoside Analysis of <i>Abeliophyllum distichum</i>

• Main Authors: Hak-Dong Lee (Author), Ji Hyun Kim (Author), Qi Qi Pang (Author), Pil-Mun Jung (Author), Eun Ju Cho (Author), Sanghyun Lee (Author)
• Format: Book
• Published: MDPI AG, 2020-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox9111148; 2076-3921
• Keywords: <i>Abeliophyllum distichum</i>; acteoside; DPPH; hydroxyl radical; HPLC/UV; O₂⁻ radical; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 9, Iss 11, p 1148 (2020)

This study determined acteoside and its content in <i>Abeliophyllum distichum</i> via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (^•OH), and O₂⁻ radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, ^•OH, and O₂⁻ radical scavenging activities of <i>A. distichum</i> leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in <i>A. distichum</i> leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in <i>A. distichum</i> leaves, which resulted in DPPH, ^•OH, and O₂⁻ radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that <i>A. distichum</i> leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.