Myocardial infarction model induced by isoproterenol in rats and potential cardiovascular protective effect of a nattokinase-containing hard capsule

• Main Authors: Van Anh Pham (Author), Ha Thai Tran (Author), Thanh Phuong Mai (Author), Lien Huong Nguyen (Author), Van Hong Nguyen (Author), Thang Huu Nguyen (Author), Son Song Bui (Author), An Van Vu (Author), Ha Thanh Do (Author), Quang Vinh Trinh (Author)
• Format: Book
• Published: Elsevier, 2023-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2667-0313; 10.1016/j.phyplu.2023.100472
• Keywords: Isoproterenol-induced myocardial infarction; Nattokinase; Rat ECG; eNOS; Anti-oxidant activity; Other systems of medicine; RZ201-999
• Source: Phytomedicine Plus, Vol 3, Iss 3, Pp 100472- (2023)

Background: TD.HK01® consists of a Japanese traditional medicine: Nattokinase (derived from soybean) and three other natural components, namely dried Hirudo medicinalis, Semen Mucuna interrupta and Huperzia carinata. Objectives: TD.HK01® is potentially aimed to the preventive treatment of cardiovascular disease (CVD) in general, and myocardial infarction (MI) in particular. Methods: In this study, a non-invasive method has been used to induce MI in rats, preceded by oral administration of TD.HK01® for 30 consecutive days to determine the potential cardiovascular effect of this pretreatment. Rats were divided into four groups: normal control group, ISO-induced group (isoproterenol (ISO), 150 mg/kg, s.c.) and two groups treated by TD.HK01® in different doses (0.31 and 0.92 g/kg, orally + ISO 150 mg/kg, s.c.). Results: TD.HK01® treated groups showed significant improvement as in abnormal electrocardiography (ECG) alteration ratio, in decreased biochemical specific markers troponin T and in decreased non-specific biochemical markers (LDH and AST) between each of TD.HK01® treated groups and ISO-induced group. The effect on vascular system was also evaluated through serum eNOS quantification and a notable increase in an anti-oxidative enzyme catalase. All three groups, except the normal control one, had pathological lesions in histopathology in different levels - modest injuries in treated groups and more severe in ISO-induced group. Conclusions: It is concluded on the basis of the findings that isoproterenol induced MI in all three groups and TD.HK01® did have its potential protective effect in vivo on cardiovascular system.