Subculture of Germ Cell-Derived Colonies with GATA4-Positive Feeder Cells from Neonatal Pig Testes

• Main Authors: Kyung Hoon Lee (Author), Won Young Lee (Author), Jin Hoi Kim (Author), Chan Kyu Park (Author), Jeong Tae Do (Author), Jae Hwan Kim (Author), Young Suk Choi (Author), Nam Hyung Kim (Author), Hyuk Song (Author)
• Format: Book
• Published: Hindawi Limited, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Enrichment of spermatogonial stem cells is important for studying their self-renewal and differentiation. Although germ cell-derived colonies (GDCs) have been successfully cultured from neonatal pig testicular cells under 31°C conditions, the short period of in vitro maintenance (<2 months) limit...