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Characterization of Extended-Spectrum β-Lactamase-Producing and AmpC β-Lactamase-Producing <i>Enterobacterales</i> Isolated from Companion Animals in Korea

The emergence of extended-spectrum cephalosporin (ESC)-resistant Gram-negative bacteria is of great concern in both human and veterinary medicine. The aim of this study was to investigate ESC-resistant bacterial isolates from companion animals in South Korea between 2017 and 2019. Isolates with ESC...

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Main Authors: Se Ra Shin (Author), Seong Mi Noh (Author), Woo Kyung Jung (Author), Sook Shin (Author), Young Kyung Park (Author), Dong Chan Moon (Author), Suk-Kyung Lim (Author), Yong Ho Park (Author), Kun Taek Park (Author)
Format: Book
Published: MDPI AG, 2021-03-01T00:00:00Z.
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Summary:The emergence of extended-spectrum cephalosporin (ESC)-resistant Gram-negative bacteria is of great concern in both human and veterinary medicine. The aim of this study was to investigate ESC-resistant bacterial isolates from companion animals in South Korea between 2017 and 2019. Isolates with ESC resistance genes, which were identified by PCR, were assessed for genetic relatedness by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). In total, 91 ESC-resistant <i>Escherichia coli, Klebsiella</i> spp., <i>Serratia</i> spp., and <i>Enterobacter cloacae</i> isolates harbored the <i>bla</i><sub>TEM</sub> gene. Among other ESC resistance genes, <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>CIT</sub>, and <i>bla</i><sub>CTX-M-55</sub> were predominantly detected in <i>E. coli</i> isolates, whereas <i>bla</i><sub>SHV</sub> and <i>bla</i><sub>DHA</sub> were more frequently detected in <i>Klebsiella pneumoniae</i> isolates. In addition, all <i>bla</i><sub>EBC</sub>-positive isolates were classified as <i>E. cloacae</i>. From the MLST results, <i>bla</i><sub>CTX-M-9</sub>-carrying ST131, <i>bla</i><sub>CIT</sub>-carrying ST405, and <i>bla</i><sub>CTX-M-1</sub>-carrying ST3285 strains were dominant among <i>E. coli</i> isolates. ST273 and ST275 strains harboring <i>bla</i><sub>SHV</sub> were frequently detected in <i>K. pneumoniae</i> isolates. Various sequence types were obtained in <i>E. cloacae</i> and <i>Klebsiella oxytoca</i> isolates. All isolates demonstrated unique PFGE profiles (<57-98% similarity) and were unlikely to be derived from a single clone. The present study reveals the presence and wide genetic distribution of ESC-resistant bacterial species in South Korean companion animals.
Item Description:10.3390/antibiotics10030249
2079-6382

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