Augmenting Rice Defenses: Exogenous Calcium Elevates GABA Levels Against WBPH Infestation

• Main Authors: Rahmatullah Jan (Author), Sajjad Asaf (Author), Lubna (Author), Muhammad Farooq (Author), Saleem Asif (Author), Zakirullah Khan (Author), Jae-Ryoung Park (Author), Eun-Gyeong Kim (Author), Yoon-Hee Jang (Author), Kyung-Min Kim (Author)
• Format: Book
• Published: MDPI AG, 2024-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox13111321; 2076-3921
• Keywords: calcium; gamma-aminobutyric acid; white-backed planthopper; oxidative stress; antioxidant activity; hormones; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 13, Iss 11, p 1321 (2024)

This study investigates the impact of exogenous calcium and gamma-aminobutyric acid (GABA) supplementation on rice growth and stress tolerance under white-backed planthopper (WBPH) infestation. We evaluated several phenotypic traits, including shoot/root length, leaf width, tiller number, panicle length, and relative water content, alongside physiological markers such as oxidative stress indicators, antioxidant enzymes activities, hormonal levels, and amino acids biosynthesis. Our results indicate that WBPH stress significantly reduces growth parameters but calcium and GABA supplementation markedly enhance shoot length (by 26% and 36%) and root length (by 38% and 64%), respectively, compared to WBPH-infested plants. Both supplementations also reduced oxidative stress, as evidenced by decreased H₂O₂ and O₂^•− levels and a lower electrolyte leakage. Notably, calcium and GABA treatments increased antioxidant enzyme activities, with GABA boosting catalase (CAT) activity by 800%, peroxidase (POD) by 144%, and superoxide dismutase (SOD) by 62% under WBPH stress. Additionally, calcium and GABA enhanced the accumulation of stress hormones (abscisic acid ABA) and salicylic acid (SA) and promoted stomatal closure, contributing to improved water conservation. This study reveals that calcium regulates the GABA shunt pathway, significantly increasing GABA and succinate levels in both root and shoot. Furthermore, calcium and GABA supplementation enhance the biosynthesis of key amino acids and improve ion homeostasis, particularly elevating calcium (Ca), iron (Fe), and magnesium (Mg) levels under WBPH stress. Overall, this study highlights the potential of exogenous calcium and GABA as effective strategies for enhancing rice plant tolerance to WBPH infestation by modulating various physiological and biochemical mechanisms. Further research is warranted to fully elucidate the underlying mechanisms of action.