Purification of Phenolic Compounds from <i>Camellia polyodonta</i> Flower: Composition Analysis, Antioxidant Property, and Hypolipidemic Activity In Vitro and In Vivo

• Main Authors: Zhuoya Xiang (Author), Li Liu (Author), Zhou Xu (Author), Qingbo Kong (Author), Heng Liang (Author), Shiling Feng (Author), Tao Chen (Author), Lijun Zhou (Author), Hongyu Yang (Author), Chunbang Ding (Author)
• Format: Book
• Published: MDPI AG, 2024-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<i>Camellia polyodonta</i> flowers are rich sources of phenolics and less attention has been paid to their potential biological activity. This study aims to explore the crude extracts and resulting purified fractions (CPFP-I, II, III, and IV) through compositional analysis and antioxidant and hypolipidemic activities in vitro and in vivo. Among four fractions, CPFP-II contained the highest total phenolic content and flavonoid content, while CPFP-III exhibited the greatest total proanthocyanidin content. Among the 14 phenolic compounds, CPFP-II displayed the highest content of procyanidin B2, B4, and C1, whereas CPFP-III contained the highest amount of 1,2,3,6-tetragalloylglucose. The DPPH, ABTS, and FRAP assessments demonstrated a consistent trend: CPFP-II > CPFP-III > CPFP-I > CPFP-IV. In vivo experiments showed that that all four fractions significantly reduced lipid levels in hyperlipidemic <i>C. elegans</i> (<i>p</i> < 0.05), with CPFP-II exhibiting the most potent effect. Furthermore, CPFP-II effectively bound to bile acids and inhibited the enzymatic activity of pancreatic lipase in vitro. Consequently, CPFP-II should be prioritized as a promising fraction for further exploration and should provide substantial support for the feasibility of the <i>C. polyodonta</i> flower as a natural alternative.

Item Description

10.3390/antiox13060662
2076-3921