First Molecular Identification of Three Clinical Isolates of Fungi Causing Mucormycosis in Honduras
Mucormycoses are rare but serious opportunistic fungal infections caused by filamentous organisms of the order Mucorales. Here we report the first molecular identification of <i>Rhizopus</i> <i>oryzae</i> (heterotypic synonym <i>Rhizopus arrhizus</i>), <i>R....
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-04-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Mucormycoses are rare but serious opportunistic fungal infections caused by filamentous organisms of the order Mucorales. Here we report the first molecular identification of <i>Rhizopus</i> <i>oryzae</i> (heterotypic synonym <i>Rhizopus arrhizus</i>), <i>R. delemar</i>, and <i>Apophysomyces ossiformis</i> as the etiological agents of three cases of severe mucormycosis in Honduras. Conventional microbiological cultures were carried out, and DNA was extracted from both clinical samples and axenic cultures. The ITS ribosomal region was amplified and sequenced. Molecular tools are suitable strategies for diagnosing and identifying Mucorales in tissues and cultures, especially in middle-income countries lacking routine diagnostic strategies. |
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| Item Description: | 10.3390/idr14020031 2036-7449 |