Identification of IncA Plasmid, Harboring <i>bla</i>_VIM-1 Gene, in <i>S. enterica</i> Goldcoast ST358 and <i>C. freundii</i> ST62 Isolated in a Hospitalized Patient

• Main Authors: Alessandra Piccirilli (Author), Sascia Di Marcantonio (Author), Venera Costantino (Author), Omar Simonetti (Author), Marina Busetti (Author), Roberto Luzzati (Author), Luigi Principe (Author), Marco Di Domenico (Author), Antonio Rinaldi (Author), Cesare Cammà (Author), Mariagrazia Perilli (Author)
• Format: Book
• Published: MDPI AG, 2023-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In the present study, we analyzed the genome of two <i>S. enterica</i> strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of <i>C. freundii</i> TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The <i>S. enterica</i> Goldcoast ST358 (O:8 (C2-C3) serogroup), sequenced by the MiSeq Illumina system, showed the presence of β-lactamase genes (<i>bla</i>_VIM-1, <i>bla</i>_SHV-12 and <i>bla</i>_OXA-10), <i>aadA1</i>, <i>ant(2")-Ia</i>, <i>aac(6')-Iaa</i>, <i>aac(6')-Ib3</i>, <i>aac(6')-Ib-cr</i>, <i>qnrVC6</i>, <i>parC(T57S),</i> and several incompatibility plasmids. A wide variety of insertion sequences (ISs) and transposon elements were identified. In <i>C. freundii</i> TS3, these were the <i>bla</i>_VIM-1, <i>bla</i>_CMY-150, and <i>bla</i>_SHV-12, <i>aadA1</i>, <i>aac(6')-Ib3</i>, <i>aac(6')-Ib-cr</i>, <i>mph(A)</i>, <i>sul1</i>, <i>dfrA14</i>, <i>ARR-2</i>, <i>qnrVC6</i>, and <i>qnrB38</i>. IncA plasmid isolated from <i>E.coli</i>/K12 transconjugant and <i>C. freundii</i> exhibited a sequence identity >99.9%. The transfer of IncA plasmid was evaluated by conjugation experiments.

Item Description

10.3390/antibiotics12121659
2079-6382