Polysaccharopeptides derived from <it>Coriolus versicolor </it>potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells
<p>Abstract</p> <p>Background</p> <p>Polysaccharopeptide (PSP) from <it>Coriolus versicolor </it>(<it>Yunzhi</it>) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase t...
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BMC,
2010-04-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Polysaccharopeptide (PSP) from <it>Coriolus versicolor </it>(<it>Yunzhi</it>) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT).</p> <p>Methods</p> <p>We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT.</p> <p>Results</p> <p>PSP reduced cellular proliferation; inhibited cells progression through both S and G<sub>2 </sub>phase, reduced <sup>3</sup>H-thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G<sub>2 </sub>phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT.</p> <p>Conclusion</p> <p>The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells.</p> |
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| Item Description: | 10.1186/1749-8546-5-16 1749-8546 |