Ultra-High-Resolution Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry Analysis of <i>Tessaria absinthioides</i> (Hook. & Arn.) DC. (Asteraceae) and Antioxidant and Hypocholesterolemic Properties
Recently, we reported the chemical profile and the hypocholesterolemic effects of a decoction of <i>Tessaria absinthioides</i> (Hook. & Arn.) DC. (Asteraceae). In this study, we evaluated a methanolic extract (METa) instead. Metabolite profiling was conducted using ultra-high-resolut...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2023-12-01T00:00:00Z.
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| Summary: | Recently, we reported the chemical profile and the hypocholesterolemic effects of a decoction of <i>Tessaria absinthioides</i> (Hook. & Arn.) DC. (Asteraceae). In this study, we evaluated a methanolic extract (METa) instead. Metabolite profiling was conducted using ultra-high-resolution liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS), identifying thirty compounds, including flavonoids, phenolic acids, fatty acids, and phorbolesters. Antioxidant properties were assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant activity (TEAC), ferric-reducing antioxidant power (FRAP), and inhibition of lipid peroxidation in erythrocytes (ILP) assays, exhibiting robust antioxidant activity. The in vivo impact of METa on serum lipid parameters and liver X receptors (LXRs) was evaluated in a hypercholesterolemic animal model. After 14 days on a high-fat diet, male rats received either a vehicle (V) or METa<sub>100</sub>, METa<sub>200</sub> or METa<sub>500</sub> (100; 200 and 500 mg METa/kg animal, respectively) for an additional two weeks. METa<sub>500</sub> reduced total cholesterol levels (17.62%; <i>p</i> < 0.05) and all doses increased high-density lipoprotein cholesterol levels (METa<sub>100</sub>: 86.27%; METa<sub>200</sub>: 48.37%, and METa<sub>500</sub>: 29.42%; <i>p</i> < 0.0001). However, METa did not alter LXRs expression. The observed antioxidant and hypocholesterolemic properties of METa may be linked to the presence of six di-caffeoylquinic acids. These findings underscore <i>T. absinthioides</i> as a potential candidate for the treatment of metabolic disease. |
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| Item Description: | 10.3390/antiox13010050 2076-3921 |