Epitope Identification in BEFV Gene for Detecting Effective Points
Background & Objective: The most common cause of milk production loss in cattle and water buffalo is bovine ephemeral fever (BEF). Previous cases have been reported in Iran's south regions, with a low mortality rate. As a result, studying BEFV and identifying ideal epitopes for further deve...
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| Main Authors: | , , , |
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| Format: | Book |
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Fasa University of Medical Sciences,
2022-03-01T00:00:00Z.
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| Summary: | Background & Objective: The most common cause of milk production loss in cattle and water buffalo is bovine ephemeral fever (BEF). Previous cases have been reported in Iran's south regions, with a low mortality rate. As a result, studying BEFV and identifying ideal epitopes for further developing diagnostic paths is important. Materials & Methods: To investigate BEFV N protein epitopes, we collected samples, extracted and sequenced DNA, and then used the ExPaSy translate method to deduce the amino acid sequence. Various immunoinformatics techniques were used to analyze physical/chemical properties, secondary structure of protein sequences, membrane topology, antigenic property, and 3D structure. BCPRED and the DiscoTope server, respectively, predicted linear and discontinuous epitopes of BEFV N protein. Finally, the PatchDock server was used to dock peptides and antibodies. Result: Three linear epitopes and sixteen discontinuous epitopic positions were discovered. Furthermore, molecular docking between epitopes and low-binding-energy antibodies revealed that they have easy access to the immune system. Conclusion: In this study, bioinformatics techniques were used to predict epitopes of the BEFV N protein for further developing BEFV diagnostic paths. Furthermore, experimental validation is needed for these epitopes. |
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| Item Description: | 2228-5105 2783-1523 |