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Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury

Progression to renal damage by ischemia-reperfusion injury (IRI) is the result of the dysregulation of various tissue damage repair mechanisms. Anesthetic preconditioning with opioids has been shown to be beneficial in myocardial IRI models. Our main objective was to analyze the influence of pharmac...

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Main Authors: Adriana Franco-Acevedo (Author), Raquel Echavarria (Author), Bibiana Moreno-Carranza (Author), Cesar-Ivan Ortiz (Author), David Garcia (Author), Ricardo Gonzalez-Gonzalez (Author), Oscar-Kurt (Author), Eliseo Portilla-De Buen (Author), Zesergio Melo (Author)
Format: Book
Published: MDPI AG, 2020-11-01T00:00:00Z.
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Summary:Progression to renal damage by ischemia-reperfusion injury (IRI) is the result of the dysregulation of various tissue damage repair mechanisms. Anesthetic preconditioning with opioids has been shown to be beneficial in myocardial IRI models. Our main objective was to analyze the influence of pharmacological preconditioning with opioids in renal function and expression of molecules involved in tissue repair and angiogenesis. Experimental protocol includes male rats with 45 min ischemia occluding the left renal hilum followed by 24 h of reperfusion with or without 60 min preconditioning with morphine/fentanyl. We analyzed serum creatinine and renal <i>KIM-1</i> expression. We measured circulating and intrarenal VEGF. Immunohistochemistry for HIF-1 and Cathepsin D (CTD) and real-time PCR for angiogenic genes <i>HIF-1α</i>, <i>VEGF</i>, VEGF Receptor 2 (<i>VEGF-R2</i>), <i>CTD</i>, <i>CD31</i> and <i>IL-6</i> were performed. These molecules are considered important effectors of tissue repair responses mediated by the development of new blood vessels. We observed a decrease in acute renal injury mediated by pharmacological preconditioning with opioids. Renal function in opioid preconditioning groups was like in the sham control group. Both anesthetics modulated the expression of HIF-1, VEGF, VEGF-R2 and CD31. Preconditioning negatively regulated CTD. Opioid preconditioning decreased injury through modulation of angiogenic molecule expression. These are factors to consider when establishing strategies in pathophysiological and surgical processes.
Item Description:10.3390/ph13110387
1424-8247

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