Evaluation of Hepatic Detoxification Effects of <i>Enteromorpha prolifera</i> Polysaccharides against Aflatoxin B₁ in Broiler Chickens

• Main Authors: Wen-Chao Liu (Author), Yu-Ying Yang (Author), Karthika Pushparaj (Author), Balamuralikrishnan Balasubramanian (Author)
• Format: Book
• Published: MDPI AG, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Aflatoxin B₁ (AFB₁) is a major risk factor in animal feed. Seaweed (<i>Enteromorpha prolifera</i>)-derived polysaccharides (SDP) are natural antioxidants with multiple biological functions, which may have an in vivo detoxification effect on AFB₁. The current study aimed to evaluate whether SDP could mitigate AFB₁-induced hepatotoxicity in broilers. A total of 216 chickens (male, 5 weeks old) were randomly allocated to three groups with differing feeding patterns, lasting 4 weeks: (1) control group (CON, fed a basal diet); (2) AFB₁ group (fed a basal diet mixed with 0.1 mg/kg AFB₁); and (3) AFB₁ + SDP group (AFB₁ group + 0.25% SDP). The results showed that dietary SDP improved the liver function-related biochemical indicators in serum, and reversed the increase in relative liver weight, hepatic apoptosis and histological damage of broilers exposed to AFB₁. SDP treatment also reduced the activity and mRNA expression of phase I detoxification enzymes, while increasing the activity and mRNA expression of phase II detoxification enzymes in the livers of AFB₁-exposed broilers, which was involved in the activation of p38 mitogen-activated protein kinase (p38MAPK)/nuclear factor erythroid 2-related factor 2 (Nrf2) signaling. In conclusion, dietary SDP alleviated AFB₁-induced liver injury of broilers through inhibiting phase I detoxification enzymes and upregulating p38MAPK/Nrf2-mediated phase II detoxification enzymes pathway.

Item Description

10.3390/antiox11091757
2076-3921