Highly sensitive trivalent copper chelateâluminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples
A simple, fast and sensitive capillary electrophoresis (CE) strategy combined with chemiluminescence (CL) detection for analysis of ofloxacin (OF) enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD) was used as the chiral additive being added into the running buffer o...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2014-12-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A simple, fast and sensitive capillary electrophoresis (CE) strategy combined with chemiluminescence (CL) detection for analysis of ofloxacin (OF) enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD) was used as the chiral additive being added into the running buffer of luminolâdiperiodatocuprate (III) (K5[Cu(HIO6)2], DPC) chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (S/N=3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010â100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5â110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines. Keywords: Ofloxacin, Chiral analysis, Sulfonated-β-CD, Capillary electrophoresis, Chemiluminescence |
|---|---|
| Item Description: | 2095-1779 10.1016/j.jpha.2014.05.004 |