Whole-Genome Characterisation of ESBL-Producing <i>E. coli</i> Isolated from Drinking Water and Dog Faeces from Rural Andean Households in Peru

• Main Authors: Maria Luisa Medina-Pizzali (Author), Apoorva Venkatesh (Author), Maribel Riveros (Author), Diego Cuicapuza (Author), Gabriela Salmon-Mulanovich (Author), Daniel Mäusezahl (Author), Stella M. Hartinger (Author)
• Format: Book
• Published: MDPI AG, 2022-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antibiotics11050692; 2079-6382
• Keywords: phylogenomic analysis; one health; ESBL-producing <i>E. coli</i>; carbapenem resistance; whole-genome sequencing; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 11, Iss 5, p 692 (2022)

<i>E. coli</i> that produce extended-spectrum β-lactamases (ESBLs) are major multidrug-resistant bacteria. In Peru, only a few reports have characterised the whole genome of ESBL enterobacteria. We aimed to confirm the identity and antimicrobial resistance (AMR) profile of two ESBL isolates from dog faeces and drinking water of rural Andean households and determine serotype, phylogroup, sequence type (ST)/clonal complex (CC), pathogenicity, virulence genes, ESBL genes, and their plasmids. To confirm the identity and AMR profiles, we used the VITEK^®2 system. Whole-genome sequencing (WGS) and bioinformatics analysis were performed subsequently. Both isolates were identified as <i>E. coli</i>, with serotypes -:H46 and O9:H10, phylogroups E and A, and ST/CC 5259/- and 227/10, respectively. The isolates were ESBL-producing, carbapenem-resistant, and not harbouring carbapenemase-encoding genes. Isolate 1143 ST5259 harboured the <i>astA</i> gene, encoding the EAST₁ heat-stable toxin. Both genomes carried ESBL genes (<i>bla</i>_EC-15, <i>bla</i>_CTX-M-8, and <i>bla</i>_CTX-M-55). Nine plasmids were detected, namely IncR, IncFIC(FII), IncI, IncFIB(AP001918), Col(pHAD28), IncFII, IncFII(pHN7A8), IncI1, and IncFIB(AP001918). Finding these potentially pathogenic bacteria is worrisome given their sources and highlights the importance of One-Health research efforts in remote Andean communities.