Antioxidant Power on Dermal Cells by Textiles Dyed with an Onion (<i>Allium cepa</i> L.) Skin Extract

• Main Authors: Claudia Volpi (Author), Desirée Bartolini (Author), Virginia Brighenti (Author), Francesco Galli (Author), Matteo Tiecco (Author), Federica Pellati (Author), Catia Clementi (Author), Roccaldo Sardella (Author)
• Format: Book
• Published: MDPI AG, 2021-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In this study, the phenol loading and antioxidant activity of wool yarn prepared with the aqueous extract of onion (<i>Allium cepa</i> L.) skin was enhanced by implementing the dyeing process with the use of alum as a mordant. Spectrophotometric and chromatographic methods were applied for the characterization of polyphenolic substances loaded on the wool yarn. The antioxidant/anti-inflammatory properties were evaluated by determining the level of intra- and extra-cellular reactive oxygen species (ROS) production in keratinocytes and dermal fibroblasts pre-treated with lipopolysaccharide put in contact with artificial sweat. An elevated dye uptake on wool was observed for the pre-mordanted sample, as demonstrated by high absorbance values in the UV-Visible spectral range. Chromatographic results showed that protocatechuic acid and its glucoside were the main phenolic acid released in artificial sweat by the wool yarns, while quercetin-4'-glucoside and its aglycone quercetin were more retained. The extract released from the textile immersed in artificial sweat showed a significant reducing effect on the intra-and extracellular ROS levels in the two cell lines considered. Cytofluorimetric analyses demonstrated that the selected mordant was safe at the concentration used in the dyeing procedure. Therefore, alum pre-mordanted textiles dyed with onion-skin extracts may represent an interesting tool against skin diseases.

Item Description

10.3390/antiox10111655
2076-3921