<b>In vitro antiherpetic activity of a chemically sulfated galactomannan from Leucaena leucocephala seeds</b>

• Main Author: LUCY ONO (Author)
• Format: Book
• Published: São Paulo State University (UNESP), 2010-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1808-4532; 2179-443X
• Keywords: Leucaena leucocephala; Galactomannan; Sulfation; HSV-1; Antiviral activity; Pharmaceutical industry; HD9665-9675; Pharmacy and materia medica; RS1-441
• Source: Revista de Ciências Farmacêuticas Básica e Aplicada, Vol 31, Iss 2, Pp 165-170 (2010)

<p align="justify">A chemically sulfated galactomannan (LLS-2) was obtained, starting from the polysaccharide extracted from seeds of Leucaena leucocephala, which presented 15.2% sulfate and a degree of sulfation of 0.60, and its effect on the replication of Herpes simplex virus 1 (HSV-1) in Vero cells was investigated. LLS-2 showed a 93.7% inhibition of the HSV-1 replication at a concentration of 2.5 micrograms/ml, when added during its adsorption step, evaluated by the plaque reduction method, and a selectivity index higher than 1 000, suggesting that it inhibits the HSV-1 binding to the host cell, but also, in a less significant way, it showed an antiviral effect following the virus attachment step, which indicates that sulfated polysaccharides could act by more than one antiviral mechanism. <i>Keywords</i>: Leucaena leucocephala. Galactomannan. Sulfation. HSV-1; Antiviral activity <b>RESUMO</b> Obteve-se uma galactomanana quimicamente sulfatada (LLS-2) a partir de polissacarídeo extraído de sementes de Leucaena leucocephala, a qual apresentou 15.2% de sulfato e grau de derivatização de 0,60, e, seu efeito antiviral sobre a replicação do vírus Herpes simplex tipo 1 (HSV-1) em células Vero foi avaliado pela metodologia de redução do número de unidades formadoras de placas. LLS-2 apresentou 93.7% de inibição da replicação viral à concentração de 2,5 μg/ml, quando adicionado durante as etapas iniciais de replicação, com um índice de seletividade maior que 1.000, sugerindo que LLS-2 inibe a ligação de HSV-1 às células hospedeiras. <i>Palavras-chave</i>: Leucaena leucocephala. Galactomanana. Sulfatação. HSV-1. Atividade antiviral.</p>