Effect of Different Concentration of Ri Plasmid on Division of Electrotreated Cell Suspension Culture derived from Stem Callus of Vicia faba L. Plants Embedded in Agar Multiple Drops

Samples of cell suspension represents seven different densities were exposed to three electrotreatment 40, 50, 60 volt and for a period of time 50, 100 msec then the densities were mixed with different concentration of Ri plasmid 25, 50, 75 Microliter. The electrotreatment samples cultured by embedd...

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Main Authors: Sahla Zedan (Author), Mozahim Al-Malah (Author)
Format: Book
Published: College of Education for Pure Sciences, 2021-08-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Sahla Zedan  |e author 
700 1 0 |a Mozahim Al-Malah  |e author 
245 0 0 |a Effect of Different Concentration of Ri Plasmid on Division of Electrotreated Cell Suspension Culture derived from Stem Callus of Vicia faba L. Plants Embedded in Agar Multiple Drops 
260 |b College of Education for Pure Sciences,   |c 2021-08-01T00:00:00Z. 
500 |a 1812-125X 
500 |a 2664-2530 
500 |a 10.33899/edusj.2021.168834 
520 |a Samples of cell suspension represents seven different densities were exposed to three electrotreatment 40, 50, 60 volt and for a period of time 50, 100 msec then the densities were mixed with different concentration of Ri plasmid 25, 50, 75 Microliter. The electrotreatment samples cultured by embedding in to agar drops in solid-liquid cultures.<br /> Results proved that the electrotreatment enhanced division of cell suspension and caused a clear increase of microcolonies, the total number of colonies reached 3276 when suspension culture was exposed to 50v / 100 msec., these colonies developed to microcalli and the percentage was 90%. The Callus developed from these colonies was light green in color and friable in texture. 
546 |a AR 
546 |a EN 
690 |a vicia faba 
690 |a cell suspension 
690 |a cocultivation 
690 |a agrobacterium rhizogenes 
690 |a Education 
690 |a L 
690 |a Science (General) 
690 |a Q1-390 
655 7 |a article  |2 local 
786 0 |n مجلة التربية والعلم, Vol 30, Iss 3, Pp 225-235 (2021) 
787 0 |n https://edusj.mosuljournals.com/article_168834_74e222a0871e49260d8c5b1e4cf2f2fb.pdf 
787 0 |n https://doaj.org/toc/1812-125X 
787 0 |n https://doaj.org/toc/2664-2530 
856 4 1 |u https://doaj.org/article/2b4e81c389094f0e8b5eb16cb6c872c5  |z Connect to this object online.