Antioxidant, cholesterol lowering activity, and analysis of Caesalpinia bonducella seeds extract
Tombili plant (Caesalpinia bonducella) belongs to the family of Fabaceae. The seed extract of tombili has been empirically used as a traditional medicine. The purpose of this research was to fractionate tombili seed extract and test their antioxidant and cholesterol lowering activities. Extraction w...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Book |
| Published: |
Pensoft Publishers,
2023-01-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Tombili plant (Caesalpinia bonducella) belongs to the family of Fabaceae. The seed extract of tombili has been empirically used as a traditional medicine. The purpose of this research was to fractionate tombili seed extract and test their antioxidant and cholesterol lowering activities. Extraction was made into fractions using n-hexane, ethyl acetate, and methanol as a solvent. The chemical compound of the ethyl acetate fraction was analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). Antioxidant activity was tested using the DPPH method. The highest antioxidant activity was shown in ethyl acetate fraction with an IC50 value of 86.153 μg/mL. The second was the methanol fraction with an IC50 value of 94.053 μg/mL, and the third was the n-hexane fraction with an IC50 value of 100.933 μg/mL. The cholesterol lowering activity analysis showed that all fractions could inhibit cholesterol. The highest anti-cholesterol activity shown in ethyl acetate fraction with the concentration of 600 μg/mL can inhibit 81.5% of the cholesterol activity. The LC-MS/MS analysis showed that the ethyl acetate fraction contained glucoside, homoplantaginin, and vernolic acid compounds. |
|---|---|
| Item Description: | 10.3897/pharmacia.70.e96817 2603-557X |