Characterization of a Novel Bacteriophage Henu2 and Evaluation of the Synergistic Antibacterial Activity of Phage-Antibiotics
<i>Staphylococcus aureus</i> phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43,513 bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Format: | Book |
Published: |
MDPI AG,
2021-02-01T00:00:00Z.
|
Subjects: | |
Online Access: | Connect to this object online. |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
LEADER | 00000 am a22000003u 4500 | ||
---|---|---|---|
001 | doaj_2e10fd2cb1be4e4f9c0b920dfe681036 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Xianghui Li |e author |
700 | 1 | 0 | |a Tongxin Hu |e author |
700 | 1 | 0 | |a Jiacun Wei |e author |
700 | 1 | 0 | |a Yuhua He |e author |
700 | 1 | 0 | |a Abualgasim Elgaili Abdalla |e author |
700 | 1 | 0 | |a Guoying Wang |e author |
700 | 1 | 0 | |a Yanzhang Li |e author |
700 | 1 | 0 | |a Tieshan Teng |e author |
245 | 0 | 0 | |a Characterization of a Novel Bacteriophage Henu2 and Evaluation of the Synergistic Antibacterial Activity of Phage-Antibiotics |
260 | |b MDPI AG, |c 2021-02-01T00:00:00Z. | ||
500 | |a 10.3390/antibiotics10020174 | ||
500 | |a 2079-6382 | ||
520 | |a <i>Staphylococcus aureus</i> phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43,513 bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage Henu2 belongs to the family <i>Siphoviridae</i> and possesses an isometric head (63 nm in diameter). The latent time and burst size of Henu2 were approximately 20 min and 7.8 plaque forming unit (PFU)/infected cells. The Henu2 maintained infectivity over a wide range of temperature (10-60 °C) and pH values (4-12). Phylogenetic and comparative genomic analyses indicate that <i>Staphylococcus aureus</i> phage Henu2 should be a new member of the family of <i>Siphoviridae</i> class-II. In this paper, Phage Henu2 alone exhibited weak inhibitory activity on the growth of <i>S. aureus</i>. However, the combination of phage Henu2 and some antibiotics or oxides could effectively inhibit the growth of <i>S. aureus</i>, with a decrease of more than three logs within 24 h in vitro. These results provide useful information that phage Henu2 can be combined with antibiotics to increase the production of phage Henu2 and thus enhance the efficacy of bacterial killing. | ||
546 | |a EN | ||
690 | |a <i>Staphylococcus aureus</i> | ||
690 | |a bacteriophage | ||
690 | |a phage-antibiotic synergy | ||
690 | |a Therapeutics. Pharmacology | ||
690 | |a RM1-950 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Antibiotics, Vol 10, Iss 2, p 174 (2021) | |
787 | 0 | |n https://www.mdpi.com/2079-6382/10/2/174 | |
787 | 0 | |n https://doaj.org/toc/2079-6382 | |
856 | 4 | 1 | |u https://doaj.org/article/2e10fd2cb1be4e4f9c0b920dfe681036 |z Connect to this object online. |