Characterization of a Novel Bacteriophage Henu2 and Evaluation of the Synergistic Antibacterial Activity of Phage-Antibiotics

<i>Staphylococcus aureus</i> phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43,513 bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage...

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Main Authors: Xianghui Li (Author), Tongxin Hu (Author), Jiacun Wei (Author), Yuhua He (Author), Abualgasim Elgaili Abdalla (Author), Guoying Wang (Author), Yanzhang Li (Author), Tieshan Teng (Author)
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Published: MDPI AG, 2021-02-01T00:00:00Z.
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100 1 0 |a Xianghui Li  |e author 
700 1 0 |a Tongxin Hu  |e author 
700 1 0 |a Jiacun Wei  |e author 
700 1 0 |a Yuhua He  |e author 
700 1 0 |a Abualgasim Elgaili Abdalla  |e author 
700 1 0 |a Guoying Wang  |e author 
700 1 0 |a Yanzhang Li  |e author 
700 1 0 |a Tieshan Teng  |e author 
245 0 0 |a Characterization of a Novel Bacteriophage Henu2 and Evaluation of the Synergistic Antibacterial Activity of Phage-Antibiotics 
260 |b MDPI AG,   |c 2021-02-01T00:00:00Z. 
500 |a 10.3390/antibiotics10020174 
500 |a 2079-6382 
520 |a <i>Staphylococcus aureus</i> phage Henu2 was isolated from a sewage sample collected in Kaifeng, China, in 2017. In this study, Henu2, a linear double-stranded DNA virus, was sequenced and found to be 43,513 bp long with 35% G + C content and 63 putative open reading frames (ORFs). Phage Henu2 belongs to the family <i>Siphoviridae</i> and possesses an isometric head (63 nm in diameter). The latent time and burst size of Henu2 were approximately 20 min and 7.8 plaque forming unit (PFU)/infected cells. The Henu2 maintained infectivity over a wide range of temperature (10-60 °C) and pH values (4-12). Phylogenetic and comparative genomic analyses indicate that <i>Staphylococcus aureus</i> phage Henu2 should be a new member of the family of <i>Siphoviridae</i> class-II. In this paper, Phage Henu2 alone exhibited weak inhibitory activity on the growth of <i>S. aureus</i>. However, the combination of phage Henu2 and some antibiotics or oxides could effectively inhibit the growth of <i>S. aureus</i>, with a decrease of more than three logs within 24 h in vitro. These results provide useful information that phage Henu2 can be combined with antibiotics to increase the production of phage Henu2 and thus enhance the efficacy of bacterial killing. 
546 |a EN 
690 |a <i>Staphylococcus aureus</i> 
690 |a bacteriophage 
690 |a phage-antibiotic synergy 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Antibiotics, Vol 10, Iss 2, p 174 (2021) 
787 0 |n https://www.mdpi.com/2079-6382/10/2/174 
787 0 |n https://doaj.org/toc/2079-6382 
856 4 1 |u https://doaj.org/article/2e10fd2cb1be4e4f9c0b920dfe681036  |z Connect to this object online.