Evaluation of Cyto - and Genotoxic Influence of Lanthanum Dioxide Nanoparticles on Human Liver Cells

• Main Authors: Saba M. Alsubaie (Author), Daoud Ali (Author), Bader O. Almutairi (Author), Rafa Almeer (Author), Saud Alarifi (Author)
• Format: Book
• Published: SAGE Publishing, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1559-3258; 10.1177/15593258221128428
• Keywords: Therapeutics. Pharmacology; RM1-950
• Source: Dose-Response, Vol 20 (2022)

Inorganic nanoparticles are representing an emerging paradigm in molecular imaging probe design. We have determined lanthanum oxide nanoparticles (La 2 O 3 NPs)-induced toxicity on human livers cells for 48 hrs. Before exposure to La 2 O 3 NPs, the size and shape of NPs were confirmed by transmission electron microscope. It was found at 32 ±1.6 nm with a sheet-like morphological structure. The viability of CHANG and HuH-7 cells was reduced as the concentration of La 2 O 3 NPs increased. HuH-7 cells were more sensitive than CHANG cells to La 2 O 3 NPs. We observed production of intracellular ROS in HuH-7 cells was more than CHANG cells and the LPO level was more in CHANG cells than in HuH-7 cells at 50 μg/ml of La 2 O 3 NPs. Glutathione was decreased and catalase was increased at 50 μg/ml of La 2 O 3 NPs. More apoptotic and necrotic cells were observed at 300 μg/ml in HuH-7 cells FACS. DNA damage was observed by the SGCE test and more DNA damage was found in CHANG cells than HuH-7 cells at 300 μg/ml La 2 O 3 NPs over 48 hrs. Thus, study warrants the application of La 2 O 3 NPs in daily life and provides vital information about the toxicity of La 2 O 3 NPs.