The design and evaluation of the antimicrobial activity of a novel conjugated penta-ultrashort antimicrobial peptide in combination with conventional antibiotics against sensitive and resistant strains of S. aureus and E. coli.

Background and purpose: Antimicrobial resistance still constitutes a major health concern to the global human population. The development of new classes of antimicrobial agents is urgently needed to thwart the continuous emergence of highly resistant microbial pathogens. Experimental approach: In th...

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Main Authors: Rula Darwish (Author), Ammar Almaaytah (Author), Ali Salama (Author)
Format: Book
Published: Wolters Kluwer Medknow Publications, 2022-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Rula Darwish  |e author 
700 1 0 |a Ammar Almaaytah  |e author 
700 1 0 |a Ali Salama  |e author 
245 0 0 |a The design and evaluation of the antimicrobial activity of a novel conjugated penta-ultrashort antimicrobial peptide in combination with conventional antibiotics against sensitive and resistant strains of S. aureus and E. coli. 
260 |b Wolters Kluwer Medknow Publications,   |c 2022-01-01T00:00:00Z. 
500 |a 1735-5362 
500 |a 1735-9414 
500 |a 10.4103/1735-5362.359429 
520 |a Background and purpose: Antimicrobial resistance still constitutes a major health concern to the global human population. The development of new classes of antimicrobial agents is urgently needed to thwart the continuous emergence of highly resistant microbial pathogens. Experimental approach: In this study, we have rationally designed a novel conjugated ultrashort antimicrobial peptide. The peptide named naprolyginine was challenged against representative strains of wild-type and multidrug-resistant bacteria individually or in combination with individual antibiotics by employing standard antimicrobial and checkerboard assays. Findings / Results: Our results displayed that the peptide exhibits potent synergistic antimicrobial activity against resistant strains of gram-positive and gram-negative bacteria when combined with individual antibiotics. Additionally, the peptide was evaluated for its hemolytic activity against human red blood cells and displayed negligible toxicity. Conclusion and implications: Naprolyginine could prove to be a promising candidate for antimicrobial drug development. 
546 |a EN 
690 |a antimicrobial peptides; bacteria; drug design; hemolysis; naproxen 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Research in Pharmaceutical Sciences, Vol 17, Iss 6, Pp 612-620 (2022) 
787 0 |n http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=6;spage=612;epage=620;aulast=Darwish 
787 0 |n https://doaj.org/toc/1735-5362 
787 0 |n https://doaj.org/toc/1735-9414 
856 4 1 |u https://doaj.org/article/2e94ecbd53d34b439b18f21523e70a89  |z Connect to this object online.