Effect of divalent metal ions on the activity and stability of β-galactosidase isolated from Kluyveromyces lactis

• Main Authors: Paulo Roberto Adalberto (Author), Antonio Carlos Massabni (Author), Eleonora Cano Carmona (Author), Antônio José Goulart (Author), Daniela Parreira Marques (Author), Rubens Monti (Author)
• Format: Book
• Published: São Paulo State University (UNESP), 2010-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this study, it was demonstrated that β-galactosidase can be deactivated and reactivated with EDTA and divalent metal ions. The enzyme was deactivated after 20 minutes in EDTA solution. Maximal deactivation at the lowest EDTA concentration (10-3 mol.L-1) occurred in the presence of Tris-HCl buffer...