Effect of divalent metal ions on the activity and stability of β-galactosidase isolated from Kluyveromyces lactis
In this study, it was demonstrated that β-galactosidase can be deactivated and reactivated with EDTA and divalent metal ions. The enzyme was deactivated after 20 minutes in EDTA solution. Maximal deactivation at the lowest EDTA concentration (10-3 mol.L-1) occurred in the presence of Tris-HCl buffer...
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| Main Authors: | , , , , , |
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| Format: | Book |
| Published: |
São Paulo State University (UNESP),
2010-12-01T00:00:00Z.
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