Transformation analysis of oral epithelial dysplasia to carcinoma in-situ and squamous cell carcinoma by p53 expression and gene mutations

• Main Author: Mei Syafriadi (Author)
• Format: Book
• Published: Universitas Airlangga, 2009-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1978-3728; 2442-9740; 10.20473/j.djmkg.v42.i2.p70-75
• Keywords: dysplasia; carcinoma in-situ; squamous cell carcinoma; mutation; p53; Dentistry; RK1-715
• Source: Dental Journal, Vol 42, Iss 2, Pp 70-75 (2009)

<span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: It is known that oral squamous epithelial dysplasia (SED) and carcinoma in-situ (CIS) are precancerous lesion </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and it could transform to squamous cell carcinoma (SCC). We had reported p53-Protein Over-Expression and Gene Mutational of </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Oral CIS, such as basaloid, verrucous, and acanthothic/atrophic types, but demarcated between SED to CIS and CIS to SCC and how </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>their transformation is still unclear. It is considered that their molecular behavior related one another. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: To understand the </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>molecular behavior of them we examined p53 exon 5-8 gene mutation and their protein expression in the sequential cases. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Using 10 cases formalin-fixed paraffin sections that composed SED appearance, CIS and SCC in the same case were subjected to P53 </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>immunohistochemistry. Then all cases were subjected to p53 gene mutations analysis. By laser capturing microdissection dysplasia </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>part, CIS part and SCC part were cutted, and followed by direct sequencing of PCR product for exon 5-8. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: SED p53-protein </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>over-expression in some cells, and the expression was increased to CIS and SCC. Mutational analysis for p53 gene showed that 60% </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of p53 gene mutation in CIS also found in SCC, therefore SCC had additional mutation in other exon of p53 gene. While no particular </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>mutations were found in SED part of all cases. </em><span style="font-family: TribuneBold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion</strong><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>: Carcinoma in-situ is a squamous cell carcinoma eventhough not invasive </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>yet, but squamous epithelial dysplasia is an early step to malignancy. It needs other genes examination to know any genes are involved </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>in the precancerous to cancer transformation process.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>