DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY

• Main Authors: E. G. Sannikova (Author), T. D. Mezenova (Author)
• Format: Book
• Published: Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute, 2016-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2307-9266; 2413-2241; 10.19163/2307-9266-2016-4-1(14)-85-90
• Keywords: rutin; leaves of willow triandra (salix triandra l.); thin-layer chromatography; identification; quantitative determination; «videodensitometry sorbfil»; Therapeutics. Pharmacology; RM1-950
• Source: Фармация и фармакология (Пятигорск), Vol 4, Iss 1(14), Pp 85-90 (2016)

The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1), detecting reagent - ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 μg/μl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%). The method is sensitive, linear, has a small error, and does not require expensive equipment.