Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane ( spp. Cv. Mex 69-290) Using a Temporary Immersion System

• Main Authors: Jericó J. Bello-Bello (Author), Rocío A. Chavez-Santoscoy (Author), Carlos A. Lecona-Guzmán (Author), Nina Bogdanchikova (Author), Josafhat Salinas-Ruíz (Author), Fernando Carlos Gómez-Merino (Author), Alexey Pestryakov (Author)
• Format: Book
• Published: SAGE Publishing, 2017-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Background: Hormesis is considered a dose-response phenomenon characterized by growth stimulation at low doses and inhibition at high doses. The hormetic response by silver nanoparticles (AgNPs) on in vitro multiplication of sugarcane was evaluated using a temporary immersion system. Methods: Sugarcane shoots were used as explants cultured in Murashige and Skoog medium with AgNPs at concentrations of 0, 25, 50, 100, and 200 mg/L. Shoot multiplication rate and length were used to determine hormetic response. Total content of phenolic compounds of sugarcane, mineral nutrition, and reactive oxygen species (ROS) was determined. Results: Results were presented as a dose-response curve. Stimulation phase growth was observed at 50 mg/L AgNPs, whereas inhibition phase was detected at 200 mg/L AgNPs. Mineral nutrient analysis showed changes in macronutrient and micronutrient contents due to the effect of AgNPs. Moreover, AgNPs induced ROS production and increased total phenolic content, with a dose-dependent effect. Conclusion: Results suggested that the production of ROS and mineral nutrition are key mechanisms of AgNP-induced hormesis and that phenolic accumulation was obtained as a response of the plant to stress produced by high doses of AgNPs. Therefore, small doses of AgNPs in the culture medium could be an efficient strategy for commercial micropropagation.

Item Description

1559-3258
10.1177/1559325817744945