Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatographyâtandem mass spectrometry

• Main Authors: Ruo-Wen Zhang (Author), Wen-Tao Liu (Author), Lu-Lu Geng (Author), Xiao-Hui Chen (Author), Kai-Shun Bi (Author)
• Format: Book
• Published: Elsevier, 2011-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

We described the first results of a quantitative ultra performance liquid chromatographyâtandem mass spectrometry method for a novel antimicrobial peptide (phylloseptin, PSN-1). Chromatographic separation was accomplished on a Waters bridged ethyl hybrid (BEH) C18 (50 mmÃ2.1 mm, 1.7 μm) column with acetonitrileâwater (25:75, v/v) as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 (PSN-1) and m/z 340.7/165 (Thymopentin, IS). Protein precipitation was investigated and the recovery was satisfactory (above 82%). The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 μg/mL with r>0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration. Keywords: Antimicrobial peptide, Phylloseptin, Ultra performance liquid chromatographyâtandem mass spectrometry, Pharmacokinetic

Item Description

2095-1779
10.1016/j.jpha.2011.04.001