Differential Expression of Gene Profiles in MRGX-treated Lung Cancer

• Main Authors: Kwon Yong-Kyun (Author), Lee Seung-Yeul (Author), Kang Hwan-Soo (Author), Sung Jung-Suk (Author), Cho Chong-Kwan (Author), Yoo Hwa-Seung (Author), Shin Seungjin (Author), Choi Jong-Soon (Author), Lee Yeon-Weol (Author), Jang Ik-Soon (Author)
• Format: Book
• Published: Korean Pharmacopuncture Institute, 2013-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.

Item Description

10.3831/KPI.2013.16.018
2093-6966
2234-6856