Facile Detection of Light-Controlled Radical Scavengers from Natural Products Using In Situ UV-LED NMR Spectroscopy

• Main Authors: InWha Park (Author), Goeun Park (Author), Yoojin Choi (Author), Seung-Woo Jo (Author), Hak Cheol Kwon (Author), Jin-Soo Park (Author), Jin Wook Cha (Author)
• Format: Book
• Published: MDPI AG, 2022-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox11112206; 2076-3921
• Keywords: free radical scavenger; in situ NMR spectroscopy; natural products; photoisomerization; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 11, Iss 11, p 2206 (2022)

With the recent development of chemical analysis technology, attention has been placed on natural light-sensitive compounds that exhibit photoreactivity to expand the structural diversity of natural product chemistry. Photochemical reactions that proceed via a free radical mechanism could be used to modulate the radical-scavenging ability of natural products as well as involve structural change. As the health benefits of radicals are also presented, there is a need for a controllable radical scavenging method for topical and selective application. In this study, we developed a novel acquisition and processing method to identify light-controlled radical scavengers in plant extracts and evaluate their antioxidant activity under light irradiation based on in situ UV-LED NMR spectroscopy. Using the developed method, licochalcones A and B, in which the <i>trans</i> and <i>cis</i> isomers undergo reversible photoisomerization, were selectively identified from licorice root extract, and their light-induced free radical scavenging activity was confirmed.