Radiographic and histopathologic outcomes of immature dog teeth with apical periodontitis after revascularization using propolis. An in vivo study

• Main Authors: Nelly Abdelsalam (Author), Ashraf M Abu-Seida (Author), Dalia Fayyad (Author), Hossam Tawfik (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2320-1495; 10.4103/sej.sej_178_19
• Keywords: intracanal medicament; odontoblasts; periapical pathosis; regenerative endodontics; revascularization; Dentistry; RK1-715
• Source: Saudi Endodontic Journal, Vol 10, Iss 3, Pp 199-207 (2020)

Introduction: This study aimed to assess the radiographic and histopathologic outcomes of immature dog teeth with apical periodontitis after revascularization using propolis. Materials and Methods: Periapical pathosis was induced in 48 double-rooted premolars in six dogs aged 6-9 months. The root canals were irrigated with 10 mL of 2.25% NaOCl per tooth. The teeth were divided into two groups (24 teeth each) according to the disinfection of root canals as follows: Group I (propolis group): disinfected with propolis paste and Group II (control group): without disinfectant. After 3 weeks, bleeding was induced to fill the canal spaces, the pulp chamber of the teeth was plugged with mineral trioxide aggregate, and the access cavities were sealed with glass ionomer cement. Samples were classified into three subgroups depending on the evaluation period as follows: subgroup i: 2 weeks, subgroup ii: 4 weeks, and subgroup iii: 8 weeks. Increase in the root length and root thickness and decease of apical diameter were assessed by radiography, and new hard tissue, vital tissue, and apical closure scores were assessed by histology. All data were statistically analyzed. Results: There were statistically significant differences between both groups regarding the increase in root length, increase in root thickness, decrease in apical diameter, new hard tissue, and vital tissue in all subgroups (P ≤ 0.05). There was a statistically significant difference between both groups regarding the apical closure at 8 weeks only (P < 0.05). Propolis group showed formation of cementum-like tissue along the inner aspect of root dentin and newly formed dentin layer along the inner aspect of the root with pulp-like tissue and odontoblasts. Conclusion: Propolis is capable of inducing hard-tissue deposition and soft-tissue formation inside the necrotic pulp after revascularization of immature permanent teeth.