Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection
A facile and robust RNA preparation protocol was developed by combining rolling circle transcription (RCT) with RNA cleavage by RNase H. Circular DNA with a complementary sequence was used as the template for promoter-free transcription. With the aid of a 2'-O-methylated DNA, the RCT-generated...
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| Main Authors: | , , , , |
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| Format: | Book |
| Published: |
Elsevier,
2015-01-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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